Test: HHV-6 Tissue Immunohistochemistry (IHC)
Immunohistochemistry (IHC) is useful when analyzing tissue samples for active herpesvirus infections. A tissue biopsy is taken from the patient, fixed in formalin, embedded in paraffin, and then cut into sections that are placed on slides. IHC analysis allows detection of viral antigens early in the course of the disease when cytopathic changes have not yet developed.
1. Label slides with patient name and specimen I.D. # and place in slide box.
2. Complete the test request form by clicking on the green Hospital/Institution Ordering button located in the blue box. Scan and send to email@example.com to give Coppe Laboratories notification that the sample is being sent.
3. A copy of the pathology report should be included with the sample submission. Place the pathology report, the test request form, and the slide box in a sealable bag or pouch, seal, and ship according to the listed shipping specifications.
4. Coppe Laboratories accepts shipments MONDAY THROUGH FRIDAY. Holiday closures will be posted on the home page of the Coppe website, www.coppelabs.com.New York Physicians: No approved waiver necessary. Coppe Laboratories holds a license with the NYSDOH (#PFI 9230) and is permitted to accept orders for the following tests: #3008 Powassan Virus IgG/IgM; #5000 West Nile Virus IgM; #2001 HHV 6 IHC; #20011 HHV 6 IHC (Endometrial)
Volume / Collection Type
N/A4 micron thick sections; 6 unstained slides4 micron thick sections; 6 unstained slidesRoom temperatureRoom temperature
Specimen:N/AVolume / Collection Type:4 micron thick sections; 6 unstained slidesMinimum:4 micron thick sections; 6 unstained slidesStorage:Room temperatureShipping:Room temperatureNote: Coppe Laboratories will arrange for samples to be embedded and sectioned for an additional charge.
Turn Around Time7 Business Days
Paraffin-embedded tissue sections sent for analysis are rehydrated prior to staining. A blocking step is used to block non-specific staining between the primary antibodies and the tissue. An antigen retrieval procedure may be used to enhance detection of the viral proteins. Commercial monoclonal antibodies directed toward the viral proteins of interest are then applied directly to the tissues. Labeled secondary antibodies allow the visualization of the viral protein within infected cells. One slide will be stained with Hemotoxylin & Eosin (H&E).