Test: HHV-6 Endometrial Tissue Immunohistochemistry (IHC)
Overview
Immunohistochemistry (IHC)
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Uses
Immunohistochemistry (IHC) is useful when analyzing tissue samples for active herpesvirus infections. An ENDOMETRIAL tissue biopsy is performed during Secretory Phase of normal menstrual cycle (Recommended Day 22 +/-5 days), fixed in formalin, and sent to Coppe Laboratories. The tissue is then embedded in paraffin, cut into sections, and placed on slides. IHC analysis allows detection of viral proteins.
Sample Instructions
Contact Coppe Laboratories to request an endometrial sample collection kit ahead of the scheduled biopsy. The kit contains instructions, screw-top container with formalin, test request form, and prepaid postage.
New York Physicians: No approved waiver necessary. Coppe Laboratories holds a license with the NYSDOH (#PFI 9230) and is permitted to accept orders for the following tests: #3008 Powassan Virus IgG/IgM; #2001 HHV 6 IHC; #20011 HHV 6 IHC (Endometrial) -
Specimen
Volume / Collection Type
Minimum
Storage
Shipping
N/A100 mg (1-2 pipelle segments 3mm x 20mm)50 mgRoom temperatureRoom temperature
Specimen:N/AVolume / Collection Type:100 mg (1-2 pipelle segments 3mm x 20mm)Minimum:50 mgStorage:Room temperatureShipping:Room temperature -
Turn Around Time
7 Business DaysDisease Details
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Methodology
Paraffin-embedded tissue sections sent for analysis are rehydrated prior to staining. A blocking step is used to block non-specific staining between the primary antibodies and the tissue. An antigen retrieval procedure may be used to enhance detection of the viral proteins. Commercial monoclonal antibodies directed toward the viral proteins of interest are then applied directly to the tissues. Labeled secondary antibodies allow the visualization of the viral protein within infected cells. One slide will be stained with Hemotoxylin & Eosin (H&E).