Diagnostic Test Menu

HHV-6 Endometrial Tissue Immunohistochemistry (IHC)

Test: HHV-6 Endometrial Tissue Immunohistochemistry (IHC)

Overview

Immunohistochemistry (IHC)

Testing for:
HHV-6
Sample Type:
ENDOMETRIAL Tissue in formalin
Test Code/s:
20011
Preferred Volume:
100 mg (1-2 pipelle segments 3mm x 20mm)
Minimum Required:
50 mg
Storage:
Room temperature
Shipping:
Room temperature
Turnaround Time:
7 Business Days
 
  • Uses

    Immunohistochemistry (IHC) is useful when analyzing tissue samples for active herpesvirus infections. An ENDOMETRIAL tissue biopsy is performed during Secretory Phase of normal menstrual cycle (Recommended Day 22 +/-5 days), fixed in formalin, and sent to Coppe Laboratories. The tissue is then embedded in paraffin, cut into sections, and placed on slides. IHC analysis allows detection of viral proteins.

    Sample Instructions

    Contact Coppe Laboratories to request an endometrial sample collection kit ahead of the scheduled biopsy. The kit contains instructions, screw-top container with formalin, test request form, and prepaid postage.

    New York Physicians: No approved waiver necessary. Coppe Laboratories holds a license with the NYSDOH (#PFI 9230) and is permitted to accept orders for the following tests: #3008 Powassan Virus IgG/IgM; #5000 West Nile Virus IgM; #2001 HHV 6 IHC; #20011 HHV 6 IHC (Endometrial)
  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    N/A
    100 mg (1-2 pipelle segments 3mm x 20mm)
    50 mg
    Room temperature
    Room temperature

    Specimen:   
    N/A
    Volume / Collection Type:   
    100 mg (1-2 pipelle segments 3mm x 20mm)
    Minimum:   
    50 mg
    Storage:   
    Room temperature
    Shipping:   
    Room temperature
  • Turn Around Time

    7 Business Days
  • Methodology

    Paraffin-embedded tissue sections sent for analysis are rehydrated prior to staining. A blocking step is used to block non-specific staining between the primary antibodies and the tissue. An antigen retrieval procedure may be used to enhance detection of the viral proteins. Commercial monoclonal antibodies directed toward the viral proteins of interest are then applied directly to the tissues. Labeled secondary antibodies allow the visualization of the viral protein within infected cells. One slide will be stained with Hemotoxylin & Eosin (H&E).