Test: West Nile Virus (WNV) IgM
West Nile Virus (WNV) is spread by mosquitoes. Clinical symptoms for WNV are non-specific and infections can only be diagnosed by means of laboratory testing. Laboratory diagnosis is generally accomplished by detecting WNV-specific IgM antibodies in the serum or cerebrospinal fluid (CSF). IgM antibodies are usually detectable from 3 to 8 days after onset of illness and persist for 30 to 90 days, (longer persistence has been documented.)
Sample InstructionsNote to Private Practice Physicians
Please contact Coppe Laboratories to request a sample collection kit ahead of the scheduled blood draw. The kit contains instructions, EDTA tube, test request form, and prepaid overnight postage.
1. Collect whole blood in EDTA tube labeled with patient name, date of birth, and date and time of draw. SAMPLES MUST BE DRAWN MONDAY THROUGH THURSDAY and sent overnight. If submitting plasma, process to plasma, and place in sterile vial or cryotube labeled with patient name, date of birth, and date and time of draw. Serum should be placed in sterile vial or cryotube labeled as above.
2. Place tube into a biohazard bag with an absorbent pad and seal.
3. Complete the test request form by clicking on the green Hospital/Institution Ordering button located in the blue box. Scan and send to firstname.lastname@example.org to give Coppe Laboratories notification that the sample is being sent.
4. Place the form and the sample in another sealable bag or pouch, seal, and ship according to the listed shipping specifications.
5. Send the sample using an overnight delivery service. Coppe Laboratories accepts shipments MONDAY THROUGH FRIDAY. Holiday closures will be posted on the home page of the Coppe website, www.coppelabs.com.New York Physicians: No approved waiver necessary. Coppe Laboratories holds a license with the NYSDOH (#PFI 9230) and is permitted to accept orders for the following tests: #3008 Powassan Virus IgG/IgM; #5000 West Nile Virus IgM; #2001 HHV 6 IHC; #20011 HHV 6 IHC (Endometrial)
Volume / Collection Type
N/AWB, 3.0 mL;
Plasma/Serum 1.0 mLWB, 1.0 mL;
Plasma/Serum 0.5 mLWB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 yearWB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
Specimen:N/AVolume / Collection Type:WB, 3.0 mL;
Plasma/Serum 1.0 mLMinimum:WB, 1.0 mL;
Plasma/Serum 0.5 mLStorage:WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 yearShipping:WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
Turn Around Time10 Business Days
The enzyme immunoassay (EIA) test provides a sensitive and specific, semi-quantitative assay for the detection of IgM antibodies to WNV. Highly specific viral protein is used to detect the antibodies to WNV and limit cross-reaction with other similar viruses.