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Lyme Tests – Antibodies to Borrelia

Test: Lyme Tests – Antibodies to Borrelia

Overview

  • 3001 – Lyme Borrelia Screening Test (Total Antibody [IgG/IgM] EIA)
  • 3002 – Lyme Confirmatory Tests (IgG and IgM Line Blots)
  • 3003 – Comprehensive Lyme Panel (includes both tests)
Testing for:
Lyme Disease
Sample Type:
Plasma, Whole Blood, Serum
Test Code/s:
3001, 3002, 3003
Preferred Volume:
WB, 3.0 mL;
Plasma/Serum 1.0 mL
Minimum Required:
WB, 1.0 mL;
Plasma/Serum 0.5 mL
Storage:
WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 year
Shipping:
WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
Turnaround Time:
5 Business Days
Hospital/Institution Ordering (PDF)
 

  • Uses

    The CDC currently recommends a two-step process when testing for evidence of antibodies against the Lyme disease bacteria, Borrelia. Both steps can be done using the same sample. Coppe Laboratories employs this two-step approach, while using recombinant VlsE and OspC antigens to increase test sensitivity.  The Coppe Lyme panel tests for multiple strains of Borrelia burgdorferi, the European species B. garinii and B. afzelii, and will also detect infections caused by B. miyamotoi.  Testing is recommended 4-6 weeks after initial tick bite.

    Sample Instructions

    Note to Private Practice Physicians
    Please contact Coppe Laboratories to request a sample collection kit ahead of the scheduled blood draw. The kit contains instructions, EDTA tube, test request form, and prepaid overnight postage.

    1. Collect whole blood in EDTA tube labeled with patient name, date of birth, and date and time of draw. SAMPLES MUST BE DRAWN MONDAY THROUGH THURSDAY and sent overnight. If submitting plasma, process to plasma, and place in sterile vial or cryotube labeled with patient name, date of birth, and date and time of draw. Serum should be placed in sterile vial or cryotube labeled as above.

    2. Place tube into a biohazard bag with an absorbent pad and seal.

    3. Complete the test request form by clicking on the green Hospital/Institution Ordering button located in the blue box. Scan and send to records@coppelabs.com to give Coppe Laboratories notification that the sample is being sent.

    4. Place the form and the sample in another sealable bag or pouch, seal, and ship according to the listed shipping specifications.

    5. Send the sample using an overnight delivery service. Coppe Laboratories accepts shipments MONDAY THROUGH FRIDAY. Holiday closures will be posted on the home page of the Coppe website, www.coppelabs.com.

    Methodology

    Lyme Screen – Total Antibody [IgG/IgM] Enzyme Immunoassay (EIA)

    • Includes Borrelia burgdorferi purified OspC antigens
      • Detects infections with multiple strains of Borrelia burgdorferi as well as B. garinii, B. afzelii and B. miyamotoi

    Confirmatory Test – Anti-Borrelia IgG and IgM Line Blots

    • Comprehensive array of diagnostically relevant Borrelia antigens
      • Recombinant VlsE from three genospecies: B. burgdorferi, B. garinii and B. afzelii
      • Immunogenic lipids from B. burgdorferi and B. afzelii upregulated during late stage disease
      • Native OspC from all three genospecies
    • Densitometry software is used for band analysis for objective results

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping

    N/A
    WB, 3.0 mL;
    Plasma/Serum 1.0 mL
    WB, 1.0 mL;
    Plasma/Serum 0.5 mL
    WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 year
    WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
    Specimen:   
    N/A
    Volume / Collection Type:   
    WB, 3.0 mL;
    Plasma/Serum 1.0 mL
    Minimum:   
    WB, 1.0 mL;
    Plasma/Serum 0.5 mL
    Storage:   
    WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 year
    Shipping:   
    WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs

  • Turn Around Time

    5 Business Days