Test: HHV-6A, HHV-6B PCR DNA CSF
- Detection in CSF for HHV-6A and HHV-6B
Due to the ubiquitous nature of human herpesvirus 6 (HHV-6), the use of traditional whole blood PCR methods to detect DNA in blood cells or tissue has limited value. When performed on whole blood samples, these PCR tests may detect DNA from a latent viral infection rather than an active infection associated with disease complications. In contrast, detection of high concentrations of viral DNA in cell-free samples such as plasma and cerebrospinal fluid (CSF) suggests the presence of virions or viral DNA from degenerating infected cells. Detection of herpesvirus DNA in cell-free samples is an important indicator of active tissue or central nervous system (CNS) infection.
1. Place CSF into a sterile tube or cryotube labeled with patient name, date of birth, and date and time of draw.
2. Place tube into a biohazard bag and seal.
3. Complete the test request form by clicking on the green Hospital/Institution Ordering button located in the blue box. Scan and send to firstname.lastname@example.org to give Coppe Laboratories notification that the sample is being sent.
4. Place the form and the sample in another sealable bag or pouch, seal, and ship according to the listed shipping specifications.
5. Send the sample using an overnight delivery service. Coppe Laboratories accepts shipments MONDAY THROUGH FRIDAY. Holiday closures will be posted on the home page of the Coppe website, www.coppelabs.com.
Volume / Collection Type
N/A1.0 mL0.5 mLRefrigerator, 6 days; frozen, 1 yearIf frozen, dry ice; If not previously frozen, ice packs
Specimen:N/AVolume / Collection Type:1.0 mLMinimum:0.5 mLStorage:Refrigerator, 6 days; frozen, 1 yearShipping:If frozen, dry ice; If not previously frozen, ice packs
DNA present in the cell-free sample is extracted using a commercial kit. To amplify the small amounts of relevant viral DNA present, viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.