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HHV-6A, HHV-6B PCR DNA CSF

Test: HHV-6A, HHV-6B PCR DNA CSF

Overview

  • Detection in CSF for HHV-6A and HHV-6B
Testing for:
HHV-6A / HHV-6B
Sample Type:
CSF
Test Code/s:
1105
Preferred Volume:
1.0 mL
Minimum Required:
0.5 mL
Storage:
Refrigerator, 6 days; frozen, 1 year
Shipping:
If frozen, dry ice; If not previously frozen, ice packs
 
  • Uses

    Due to the ubiquitous nature of human herpesvirus 6 (HHV-6), the use of traditional whole blood PCR methods to detect DNA in blood cells or tissue has limited value. When performed on whole blood samples, these PCR tests may detect DNA from a latent viral infection rather than an active infection associated with disease complications. In contrast, detection of high concentrations of viral DNA in cell-free samples such as plasma and cerebrospinal fluid (CSF) suggests the presence of virions or viral DNA from degenerating infected cells. Detection of herpesvirus DNA in cell-free samples is an important indicator of active tissue or central nervous system (CNS) infection.

    Sample Instructions

    1. Place CSF into a sterile tube or cryotube labeled with patient name, date of birth, and date and time of draw.
    2. Place tube into a biohazard bag and seal.
    3. Complete the test request form by clicking on the green Hospital/Institution Ordering button located in the blue box. Scan and send to records@coppelabs.com to give Coppe Laboratories notification that the sample is being sent.
    4. Place the form and the sample in another sealable bag or pouch, seal, and ship according to the listed shipping specifications.
    5. Send the sample using an overnight delivery service. Coppe Laboratories accepts shipments MONDAY THROUGH FRIDAY. Holiday closures will be posted on the home page of the Coppe website, www.coppelabs.com.

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    N/A
    1.0 mL
    0.5 mL
    Refrigerator, 6 days; frozen, 1 year
    If frozen, dry ice; If not previously frozen, ice packs

    Specimen:   
    N/A
    Volume / Collection Type:   
    1.0 mL
    Minimum:   
    0.5 mL
    Storage:   
    Refrigerator, 6 days; frozen, 1 year
    Shipping:   
    If frozen, dry ice; If not previously frozen, ice packs
  • Methodology

    DNA present in the cell-free sample is extracted using a commercial kit. To amplify the small amounts of relevant viral DNA present, viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.