Test: Anaplasma IgG / IgM IFA
Human anaplasmosis, formerly known as human granulocytic ehrlichiosis (HGE), is a bacterial disease transmitted to humans by Ixodes scapularis (blacklegged tick or deer tick), the same tick that transmits Lyme disease. Anaplasmosis is a challenging disease for healthcare providers to diagnose based on clinical presentation alone. The symptoms vary from patient to patient and can be difficult to distinguish from other tick-borne diseases, so laboratory testing is critical for a definitive diagnosis.
Sample InstructionsNote to Private Practice Physicians
Please contact Coppe Laboratories to request a sample collection kit ahead of the scheduled blood draw. The kit contains instructions, EDTA tube, test request form, and prepaid overnight postage.
1. Collect whole blood in EDTA tube labeled with patient name, date of birth, and date and time of draw. SAMPLES MUST BE DRAWN MONDAY THROUGH THURSDAY and sent overnight. If submitting plasma, process to plasma, and place in sterile vial or cryotube labeled with patient name, date of birth, and date and time of draw. Serum should be placed in sterile vial or cryotube labeled as above.
2. Place tube into a biohazard bag with an absorbent pad and seal.
3. Complete the test request form by clicking on the green Hospital/Institution Ordering button located in the blue box. Scan and send to firstname.lastname@example.org to give Coppe Laboratories notification that the sample is being sent.
4. Place the form and the sample in another sealable bag or pouch, seal, and ship according to the listed shipping specifications.
5. Send the sample using an overnight delivery service. Coppe Laboratories accepts shipments MONDAY THROUGH FRIDAY. Holiday closures will be posted on the home page of the Coppe website, www.coppelabs.com.
Volume / Collection Type
N/AWB, 3.0 mL;
Plasma/Serum 1.0 mLWB, 1.0 mL;
Plasma/Serum 0.5 mLWB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 yearWB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
Specimen:N/AVolume / Collection Type:WB, 3.0 mL;
Plasma/Serum 1.0 mLMinimum:WB, 1.0 mL;
Plasma/Serum 0.5 mLStorage:WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 yearShipping:WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
Turn Around Time10 Business Days
The gold standard serologic test for diagnosis of anaplasmosis is the indirect immunofluorescence assay (IFA). This assay uses human cells infected with Anaplasma phagocytophilum fixed on 12 well slides and incubated with diluted patient samples. If the sample is positive, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and can be visualized using a fluorescent microscope.