Diagnostic Test Menu

Anaplasma IgG / IgM IFA

Test: Anaplasma IgG / IgM IFA

Overview

Testing for:
Anaplasma
Sample Type:
Plasma, Serum, Whole Blood
Test Code/s:
3014
Preferred Volume:
WB, 3.0 mL;
Plasma/Serum 1.0 mL
Minimum Required:
WB, 1.0 mL;
Plasma/Serum 0.5 mL
Storage:
WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 year
Shipping:
WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
Turnaround Time:
10 Business Days
 
  • Uses

    Human anaplasmosis, formerly known as human granulocytic ehrlichiosis (HGE), is a bacterial disease transmitted to humans by Ixodes scapularis (blacklegged tick or deer tick), the same tick that transmits Lyme disease. Anaplasmosis is a challenging disease for healthcare providers to diagnose based on clinical presentation alone. The symptoms vary from patient to patient and can be difficult to distinguish from other tick-borne diseases, so laboratory testing is critical for a definitive diagnosis.

    Sample Instructions

    Note to Private Practice Physicians
    Please contact Coppe Laboratories to request a sample collection kit ahead of the scheduled blood draw. The kit contains instructions, EDTA tube, test request form, and prepaid overnight postage.

    1. Collect whole blood in EDTA tube labeled with patient name, date of birth, and date and time of draw. SAMPLES MUST BE DRAWN MONDAY THROUGH THURSDAY and sent overnight. If submitting plasma, process to plasma, and place in sterile vial or cryotube labeled with patient name, date of birth, and date and time of draw. Serum should be placed in sterile vial or cryotube labeled as above.

    2. Place tube into a biohazard bag with an absorbent pad and seal.

    3. Complete the test request form (found under ordering button). Scan and send to records@coppelabs.com to give Coppe Laboratories notification that the sample is being sent.

    4. Place the form and the sample in another sealable bag or pouch, seal, and ship according to the listed shipping specifications.

    5. Send the sample using an overnight delivery service. Coppe Laboratories accepts shipments MONDAY THROUGH FRIDAY. Holiday closures will be posted on the home page of the Coppe website, www.coppelabs.com.

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    N/A
    WB, 3.0 mL;
    Plasma/Serum 1.0 mL
    WB, 1.0 mL;
    Plasma/Serum 0.5 mL
    WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 year
    WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs

    Specimen:
    N/A
    Volume / Collection Type:
    WB, 3.0 mL;
    Plasma/Serum 1.0 mL
    Minimum:
    WB, 1.0 mL;
    Plasma/Serum 0.5 mL
    Storage:
    WB, Room temperature <48 hours; Plasma/Serum, Refrigerator, 6 days; frozen 1 year
    Shipping:
    WB, Room temperature; Plasma/Serum: If frozen, dry ice; if not previously frozen, ice packs
  • Turn Around Time

    10 Business Days
  • Methodology

    The gold standard serologic test for diagnosis of anaplasmosis is the indirect immunofluorescence assay (IFA). This assay uses human cells infected with Anaplasma phagocytophilum fixed on 12 well slides and incubated with diluted patient samples. If the sample is positive, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and can be visualized using a fluorescent microscope.