How to Order a Diagnostic Test

  • Test Uses and Alternative Names


    Real Time DNA PCR on Cell Free Samples (Plasma and CSF)

    Also known as:

    This test is ordered to determine the presence of viral DNA in samples like plasma and cerebrospinal fluid.  Cell free viral DNA from degenerating infected cells is an important indicator of active tissue or central nervous system infection.

    Learn more Order a Sample Test Kit

    Real Time DNA PCR on Cellular Samples (Whole Blood, Bone Marrow, Cord Blood)

    Also known as:

    This test is ordered as a preliminary screening test to determine the presence of the virus.  If it is positive, then more specific screening is warranted.

    Learn more Order a Sample Test Kit

    Reverse Transcription PCR for mRNA (Whole blood)

    Also known as:

    By measuring mRNA coding for viral late and immediate early structural genes, clinicians can distinguish an active herpesvirus infection from a latent, non-productive infection.  In chromosomally integrated HHV-6 (ciHHV-6), an inherited form of the virus with a DNA copy in every cell in the body, testing for mRNA is the only way to detect active infection or monitor response to anti-viral therapy.

    Learn more Order a Sample Test Kit

     HHV-6A and HHV-6B on Frozen Tissue 

    Also known as:

    This test is ordered to determine the presence of HHV-6A and HHV-6 B in tissue samples.

    Learn more Order a Sample Test Kit

    Tissue Immunohistochemistry (IHC) (FFPE slides)

    Also known as:

    IHC is useful when analyzing tissue samples for active herpesvirus infections.  Only cells with active infection stain positively using antibodies specific for productive viral infection.  The detection of these positively staining cells is definitive proof of active viral infection within the tissue.  IHC allows detection of viral antigens early in the course of the disease when cytopathic changes have not yet developed.

    Learn more Order a Sample Test Kit

    Lyme Tests

    Also known as:

    The recommended diagnostic lab test for Lyme disease uses a two-step approach. The first blood test is a screening test that uses enzyme-linked immunosorbent assay (EIA) to measure antibodies formed in response to the infection.  If the result from this test is positive or indeterminate, a second, more specific immunoblot blood test (on the same blood sample) is run to confirm the results. The comprehensive Lyme panel is ordered when both tests are requested regardless of the positivity of a screening test.

    Learn more Order a Sample Test Kit

    Powassan Tests

    Also known as:

    Until now, laboratory testing for Powassan virus has been limited to the Centers for Disease Control (CDC) and a few state laboratories. Coppe Laboratories has developed both a direct and indirect test for Powassan virus. Coppe Laboratories is the only reference laboratory to offer this testing.  Reverse transcription PCR and serologic testing are the preferred diagnostic tests for the Powassan virus.  The PCR will establish an acute infection where serology will provide evidence of antibody production in response to infection.

    Learn more Order a Sample Test Kit

    West Nile Virus (WNV) Test

    Also known as:

    The most effective way to diagnose WNV is through a blood test measuring IgG and IgM serology. The enzyme immunoassay (EIA) test done by Coppe Laboratories is a sensitive and specific, semi-quantitative assay for the detection of both IgM and IgG antibodies to WNV. Highly specific viral protein is used to detect the antibodies to WNV and limit cross-reaction with other similar viruses..

    Learn more Order a Sample Test Kit

     

    Allergy Tests


    Also known as:

    1. US Inhalation and Food Blood Allergy Tests for IgE

    Learn more Order a Sample Test Kit

  • Sample Kit Contents:

    1. Test Request Form (TRF)
    2. Absorbent sheet
    3. Biohazard bag
    4. Foil pouch
    5. ACD blood tube (yellow top)
    6. Label for blood tube
    7. Hair follicle/nail clipping zip lock bag (if needed)
    8. Instructions
    9. Preprinted FedEx® shipping label (attached to box)

    Following the individual specimen instructions is an appendix with additional information.

    Please contact Coppe Laboratories at 262-574-0701 if archival plasma samples need to be tested. Other specimens may be acceptable for testing.

    Instructions by Test Application


    Whole blood (or whole blood to be processed for plasma)

    Plasma tests ordered require ACD (yellow top tube) whole blood sample which will be processed by Coppe Laboratories.  Please call for information about acceptability of other collected plasma samples.

    1. Fill in the test request form completely.  Be sure to include date and time of specimen collection, physician information and signature, and test ordered.  Attach payment information.  Incomplete or inaccurate information may cause delays in testing and reporting of results.
    2. Draw blood in an ACD tube and gently invert blood tube 8-10 times.
    3. Label the blood tube with patient name, date of birth and date and time of draw.
    4. Place blood tube into biohazard bag with absorbent pad.
    5. Place the biohazard-labeled bag into the foil pouch and seal.
    6. Place the foil pouch and the test request form into the pre-labeled Coppe Laboratories box and seal with enclosed “Box Seal”.
    7. Send the pre-labeled box via Fed Ex, priority overnight shipping.
      1. Call FedEx at 1-800-463-3339 for pickup or
      2. Place test kit box directly into any FedEx drop box.

    Bone Marrow

    1. Fill in the test request form completely.  Be sure to include date and time of specimen collection, physician information and signature, and test ordered.  Attach payment information.  Incomplete or inaccurate information may cause delays in testing and reporting of results.
    2. Place 1 mL of bone marrow aspirate in a pediatric (3mL) ACD tube and gently invert blood tube 8-10 times.
    3. Label the tube with patient name, date of birth and date and time of draw.
    4. Place blood tube into biohazard bag with absorbent pad.
    5. Place the biohazard-labeled bag into the foil pouch and seal.
    6. Place the foil pouch and the test request form into the pre-labeled Coppe Laboratories box and seal with enclosed “Box Seal”.
    7. Send the pre-labeled box via Fed Ex, priority overnight shipping.
      1. Call FedEx at 1-800-463-3339 for pickup or
      2. Place test kit box directly into any FedEx drop box.

    Cord Blood

    1. Fill in the test request form completely.  Be sure to include date and time of specimen collection, physician information and signature, and test ordered.  Attach payment information.  Incomplete or inaccurate information may cause delays in testing and reporting of results.
    2. Label the cord blood specimen with patient name, date of birth and date and time of draw. An ACD tube is required. (Cord blood segments are also acceptable.)
    3. Place the cord blood tube into biohazard bag with absorbent pad.
    4. Place the biohazard-labeled bag into the foil pouch and seal.
    5. Place the foil pouch and the test request form into the pre-labeled Coppe Laboratories box and seal with enclosed “Box Seal”.
    6. Send the pre-labeled box via Fed Ex.
      1. Call FedEx at 1-800-463-3339 for pickup or
      2. Place test kit box directly into any FedEx drop box.

    CSF

    Since CSF samples will be sent refrigerated, please freeze gel ice packs for 4 hours prior to shipping.

    1. Fill in the test request form completely.  Be sure to include date and time of specimen collection, physician information and signature, and test ordered.  Attach payment information.  Incomplete or inaccurate information may cause delays in testing and reporting of results.
    2. Place CSF into a sterile, leak-proof plastic tube.
    3. Label the CSF specimen with patient name, date of birth and date and time of draw.
    4. Place tube into the biohazard bag.
    5. Place the frozen gel pack into the foil pouch.
    6. Place the biohazard-labeled bag into the foil pouch and seal.
    7. Place the foil pouch and the test request form into the pre-labeled Coppe Laboratories box and seal.
    8. Send the pre-labeled box via Fed Ex, priority overnight shipping.
      1. Call FedEx at 1-800-463-3339 for pickup or
      2. Place test kit box directly into any FedEx drop box.

    If a sample cannot be immediately shipped, it can be stored for 3 days refrigerated or 1 year frozen.  Must be shipped with dry ice if frozen or ice packets if stored refrigerated.

    Hair Follicle

    1. Fill in the test request form completely.  Be sure to include date and time of specimen collection, physician information and signature, and test ordered.  Attach payment information. Incomplete or inaccurate information may cause delays in testing and reporting of results.

    2. Explain to the patient that you will need to remove 2-3 hairs from their head.  Hair taken from combs or brushes is not an acceptable specimen.

    3. Using a tweezer or forceps, grasp the hair near the scalp and pull quickly.

    4. Check root to ensure hair follicle is attached. Lack of follicle on the hair shaft will cause the sample to be rejected.

    5. Repeat steps 3-4 twice.

    6. Place hair in 2×3 zip lock bag.

    7. Label the zip lock specimen bag with the patient first name, last name, date of birth, and specimen removal date.

    8. Place zip lock bag in biohazard bag and seal.

    9. Place biohazard bag into padded foil bag and seal.

    10. Place foil bag and completed test request form in Coppe Laboratories box and seal.

    11. Send the pre-labeled box via Fed Ex.

    1. Call Fed Ex at 1-800-463-3339 for pickup or
    2. Place test kit box directly into any Fed Ex drop box.

    Unstained slides for Immunohistochemistry

    1. Fill in the test request form completely.  Be sure to include date and time of specimen collection, physician information and signature, and test ordered.  Attach payment information. Incomplete or inaccurate information may cause delays in testing and reporting of results.
    2. Label slides with patient first name, last name, and date of birth.
    3. Package 6 unstained slides in a slide mailer, transporter or slide holder.
    4. Place slides into the biohazard-labeled bag.
    5. Place the biohazard-labeled bag into the foil pouch and seal.
    6. Place the foil pouch and the test request form into the pre-labeled Coppe Laboratories box and seal.
    7. Include a copy of the pathology report in the box.
    8. Send the pre-labeled box via Fed Ex.
      1. Call FedEx at 1-800-463-3339 for pickup or
      2. Place test kit box directly into any FedEx drop box.

    Bone Marrow

    1. Fill in the test request form completely. Be sure to include date and time of specimen collection, physician information and signature, and test ordered. Attach payment information. Incomplete or inaccurate information may cause delays in testing and reporting of results.
    2. Place 1 mL of bone marrow aspirate in a pediatric (3mL) ACD tube and gently invert blood tube 8-10 times.
    3. Label the tube with patient name, date of birth and date and time of draw.
    4. Place blood tube into biohazard bag with absorbent pad.
    5. Place the biohazard-labeled bag into the foil pouch and seal.
    6. Place the foil pouch and the test request form into the pre-labeled Coppe Laboratories box and seal with enclosed “Box Seal”.
    7. Send the pre-labeled box via Fed Ex, priority overnight shipping.
      1. Call FedEx at 1-800-463-3339 for pickup or
      2. Place test kit box directly into any FedEx drop box.