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Tissue Immunohistochemistry for HHV-6

Test: Tissue Immunohistochemistry for HHV-6

Overview

Tissue Immunohistochemistry for HHV-6 and CMV

Testing for:
HHV-6
Tested on:
Tissue FFPE
Test Codes:
2001
  • Uses

    Immunohistochemistry (IHC) is useful when analyzing tissue samples for active herpesvirus infections. A tissue biopsy is taken from the patient, fixed in formalin, embedded in paraffin wax and then cut into sections that are placed on a slide.  IHC analysis allows detection of viral antigens early in the course of the disease when cytopathic changes have not yet developed.

    Methodology

    Paraffin-embedded tissue sections sent for analysis are rehydrated prior to staining. A blocking step is used to block non-specific staining between the primary antibodies and the tissue. An antigen retrieval procedure may be used to enhance detection of the viral proteins. Commercial monoclonal antibodies directed toward the viral proteins of interest are then applied directly to the tissues. Labeled secondary antibodies allow the visualization of the viral protein within infected cells. One slide will be stained with Hemotoxylin & Eosin (H&E).

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    4-Micron serial sections on unstained slides
    6 Glass slides
    Room temperature, indefinite storage
    Room temperature

    Specimen:
    4-Micron serial sections on unstained slides
    Volume / Collection Type:
    6 Glass slides
    Storage:
    Room temperature, indefinite storage
    Shipping:
    Room temperature
  • Causes for Rejection

    • Specimens not accompanied by a requisition or incomplete requisition
    • Unlabeled specimens
    • Insufficient quantity of specimen

    Turn Around Time

    Within seven days of receipt in lab. Rush orders are available to be completed within 72 hours of receipt.

    Results

    Qualitative Results:

    Reported as Detected / Not Detected. A Coppe Laboratories pathologist confirms the results of positive and negative controls. Proper interpretation requires complete clinical, morphologic, and immunologic information as well as proper staining and antibody selection. Improper fixation and tissue processing may interfere with analysis.

    Extra Information

    Pathology Report