Test: Tissue Immunohistochemistry for CMV, HHV-6
Tissue Immunohistochemistry for HHV-6 and CMV
Immunohistochemistry (IHC) is useful when analyzing tissue samples for active herpesvirus infections. A tissue biopsy is taken from the patient, fixed in formalin, embedded in paraffin wax and then cut into sections that are placed on a slide. IHC analysis allows detection of viral antigens early in the course of the disease when cytopathic changes have not yet developed.
Paraffin-embedded tissue sections sent for analysis are rehydrated prior to staining. A blocking step is used to block non-specific staining between the primary antibodies and the tissue. An antigen retrieval procedure may be used to enhance detection of the viral proteins. Commercial monoclonal antibodies directed toward the viral proteins of interest are then applied directly to the tissues. Labeled secondary antibodies allow the visualization of the viral protein within infected cells. One slide will be stained with Hemotoxylin & Eosin (H&E).
Volume / Collection Type
4-Micron serial sections on unstained slides6 Glass slidesRoom temperature, indefinite storageRoom temperature
Specimen:4-Micron serial sections on unstained slidesVolume / Collection Type:6 Glass slidesStorage:Room temperature, indefinite storageShipping:Room temperature
Causes for Rejection
- Specimens not accompanied by a requisition or incomplete requisition
- Unlabeled specimens
- Insufficient quantity of specimen
Turn Around Time
Within four days of receipt in lab. Rush orders are available to be completed within 72 hours of receipt.
Reported as Detected / Not Detected. A Coppe Laboratories pathologist confirms the results of positive and negative controls. Proper interpretation requires complete clinical, morphologic, and immunologic information as well as proper staining and antibody selection. Improper fixation and tissue processing may interfere with analysis.