Test: Qualitative, Real-Time DNA PCR on Cellular Samples
Herpesvirus DNA PCR on cellular samples in the gold standard for early detection of a primary infection, tracking the course of infection and monitoring response to treatment. However HHV-6 DNA PCR will not differentiate HHV-6 reactivation from patients with ciHHV-6.
DNA present in the cellular sample is extracted using a commercial kit. To amplify the small amounts of relevant viral DNA present, viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.