Diagnostic Test Menu

Reverse Transcription PCR on Cellular Samples for HHV-6A and HHV-6B mRNA

Test: Reverse Transcription PCR on Cellular Samples for HHV-6A and HHV-6B mRNA

Overview

  • Reverse Transcription PCR on Cellular Samples for mRNA
  • Whole Blood, Bone Marrow and Cord Blood for HHV-6A and HHV-6B
Testing for:
HHV-6A / HHV-6B
Tested on:
Whole Blood, Cord Blood, Bone Marrow
Test Codes:
1110
  • Uses

    Reverse transcription polymerase chain reaction (RT-PCR) represents a sensitive and powerful tool for analyzing active, replicating virus by measuring viral messenger RNA (mRNA) rather than DNA. By measuring mRNA coding for viral “late and immediate early genes,” clinicians can distinguish an active herpesvirus infection from a non-productive infection. HHV-6 RT-PCR is performed on a real-time platform that increases the sensitivity of the test.

    Methodology

    The viral mRNA is extracted from the cellular sample using a commercial kit. The RT-PCR method is used to detect the expressed viral gene target by reverse transcribing the mRNA of interest into its DNA complement (cDNA). Subsequently, the newly synthesized cDNA is amplified using traditional PCR methods.

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    Whole blood
    6–10 mL collected in ACD tube
    3 mL
    Less than 48 hours from collection to arrival in lab, room temperature
    Room temperature

    Bone Marrow
    2 mL collected in pediatric ACD tube
    1 mL
    Less than 48 hours from collection to arrival in lab, room temperature
    Room temperature

    Cord Blood
    2 mL collected in pediatric ACD tube
    1 mL
    Less than 48 hours from collection to arrival in lab, room temperature
    Room temperature

    Specimen:
    Whole blood
    Volume / Collection Type:
    6–10 mL collected in ACD tube
    Minimum:
    3 mL
    Storage:
    Less than 48 hours from collection to arrival in lab, room temperature
    Shipping:
    Room temperature
    Specimen:
    Bone Marrow
    Volume / Collection Type:
    2 mL collected in pediatric ACD tube
    Minimum:
    1 mL
    Storage:
    Less than 48 hours from collection to arrival in lab, room temperature
    Shipping:
    Room temperature
    Specimen:
    Cord Blood
    Volume / Collection Type:
    2 mL collected in pediatric ACD tube
    Minimum:
    1 mL
    Storage:
    Less than 48 hours from collection to arrival in lab, room temperature
    Shipping:
    Room temperature
    Note: Specimens must be received by lab before expiration of storage times.
  • Causes for Rejection

    • Specimens submitted in the improper tube or container
    • Specimens not accompanied by a requisition or incomplete requisition
    • Unlabeled specimens
    • Leaking containers
    • Insufficient quantity of specimen
    • Improper storage
    • Failure to receive samples prior to expiration

    Turn Around Time

    Results available within five business days of specimen receipt in the lab.

    Results

    Qualitative Results

    Reported as Detected / Not Detected

    Extra Information

    This is the most effective test to distinguish productive viral infection.