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Real-Time DNA PCR on Cell-Free Sample (DNA PCR)

Test: Real-Time DNA PCR on Cell-Free Sample (DNA PCR)

Overview

  • Qualitative Detection in Plasma for HHV-6A and HHV-6B
Testing for:
HHV-6A / HHV-6B
Tested on:
Plasma
Test Codes:
1113
  • Uses

    Due to the ubiquitous nature of human herpesviruses HHV-6, HHV-7, the use of traditional whole blood PCR methods to detect DNA in blood cells or tissue has limited value. When performed on whole blood samples, these PCR tests may detect DNA from a latent viral infection rather than an active infection associated with disease complications. In contrast, detection of high concentrations of viral DNA in cell-free samples such as plasma and cerebrospinal fluid (CSF) suggests the presence of virions or viral DNA from degenerating infected cells. Detection of herpesvirus DNA in cell-free samples is an important indicator of active tissue or central nervous system (CNS) infection.

    Methodology

    DNA present in the cell-free sample is extracted using a commercial kit. To amplify the small amounts of relevant viral DNA present, viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    Whole blood to be processed to plasma by Coppe Laboratories
    6 – 10 mL collected in ACD tube
    3 mL
    Less than 48 hours from collection to arrival in lab, room temperature
    Room temperature

    Alternate Plasma: Whole blood sample collected in ACD and processed to plasma
    1 mL
    0.5 mL
    Refrigerator 6 days; frozen 1 year
    If frozen, send on dry ice. If refrigerated, send with ice packs

    Specimen:
    Whole blood to be processed to plasma by Coppe Laboratories
    Volume / Collection Type:
    6 – 10 mL collected in ACD tube
    Minimum:
    3 mL
    Storage:
    Less than 48 hours from collection to arrival in lab, room temperature
    Shipping:
    Room temperature
    Specimen:
    Alternate Plasma: Whole blood sample collected in ACD and processed to plasma
    Volume / Collection Type:
    1 mL
    Minimum:
    0.5 mL
    Storage:
    Refrigerator 6 days; frozen 1 year
    Shipping:
    If frozen, send on dry ice. If refrigerated, send with ice packs
  • Causes for Rejection

    • Specimens submitted in the improper tube or container
    • Specimens not accompanied by a requisition or incomplete requisition
    • Unlabeled specimens
    • Leaking containers
    • Insufficient quantity of specimen
    • Improper storage
    • Failure to receive samples prior to expiration

    Turn Around Time

    Results available within five business days of specimen receipt in the lab.

    Results

    Quantitative Results for Plasma Samples

    Report as Detected / Not Detected