Test: Qualitative, Real-Time DNA PCR on Hair Follicle or Nail Clippings for ciHHV-6
Hair follicles and nail clippings can be tested for presence of HHV-6 DNA to determine integration. HHV-6 DNA would only be detected in a hair follicle (or nail clipping) in the case of ciHHV-6.
Genomic DNA present in the sample is extracted using a commercial kit. Viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.