Test: Qualitative, Real-Time DNA PCR on Hair Follicle or Nail Clippings for ciHHV-6
Qualitative, Real-Time DNA PCR on Hair Follicle (preferred) or nail clippings for ciHHV-6
Hair follicles and nail clippings can be tested for presence of HHV-6 DNA to determine integration. HHV-6 DNA would only be detected in a hair follicle (or nail clipping) in the case of ciHHV-6.
Genomic DNA present in the sample is extracted using a commercial kit. Viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.
Volume / Collection Type
Preferred - Hair follicle3-4 hairs with follicleAt least 1 follicleRoom temperatureRoom temperature
Nail clippings5+ nails clipped2-3Room temperatureRoom temperature
Specimen:Preferred - Hair follicleVolume / Collection Type:3-4 hairs with follicleMinimum:At least 1 follicleStorage:Room temperatureShipping:Room temperatureSpecimen:Nail clippingsVolume / Collection Type:5+ nails clippedMinimum:2-3Storage:Room temperatureShipping:Room temperature
Causes for Rejection
- Specimens not accompanied by a requisition or incomplete requisition
- Unlabeled specimens
- Insufficient quantity of specimen
Turn Around Time
Results are available within five business days of receipt of specimen in the lab.