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Qualitative, Real-Time DNA PCR on Hair Follicle or Nail Clippings for ciHHV-6

Test: Qualitative, Real-Time DNA PCR on Hair Follicle or Nail Clippings for ciHHV-6

Overview

Qualitative, Real-Time DNA PCR on Hair Follicle (preferred) or nail clippings for ciHHV-6

Testing for:
CiHHV-6
Tested on:
Hair Follicle
Test Codes:
1109
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  • Uses

    Hair follicles and nail clippings can be tested for presence of HHV-6 DNA to determine integration. HHV-6 DNA would only be detected in a hair follicle (or nail clipping) in the case of ciHHV-6.

    Methodology

    Genomic DNA present in the sample is extracted using a commercial kit. Viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping

    Preferred - Hair follicle
    3-4 hairs with follicle
    At least 1 follicle
    Room temperature
    Room temperature
    Nail clippings
    5+ nails clipped
    2-3
    Room temperature
    Room temperature
    Specimen:
    Preferred - Hair follicle
    Volume / Collection Type:
    3-4 hairs with follicle
    Minimum:
    At least 1 follicle
    Storage:
    Room temperature
    Shipping:
    Room temperature
    Specimen:
    Nail clippings
    Volume / Collection Type:
    5+ nails clipped
    Minimum:
    2-3
    Storage:
    Room temperature
    Shipping:
    Room temperature

  • Causes for Rejection

    • Specimens not accompanied by a requisition or incomplete requisition
    • Unlabeled specimens
    • Insufficient quantity of specimen

    Turn Around Time

    Results are available within five business days of receipt of specimen in the lab.