Test: Powassan Virus Panel (Includes PCR and EIA/IFA)
The Powassan Virus Panel will include testing for both acute and chronic infections.
Direct Testing: Powassan Acute Test (PCR)
- Measurement of the actual virus RNA.
- Used in determining acute infection in those individuals who have symptoms for less than six weeks.
Indirect Testing: Powassan Serology Panel
Measurement of patient’s antibody response to the virus
- IgG and IgM antibodies specific for Powassan detected
- Test is useful for determining stage of infection including chronic disease
PCR testing: This procedure detects the presence of Powassan virus lineage I (POWV) and lineage II (DTV) genomic RNA in human blood samples.
The EIA measures IgG and IgM antibodies to members of the tick-borne encephalitis complex (TBE-C). Due to the homology between these viruses, antibodies that cross-react with the TBE virus substrate in the EIA kit are found in samples from patients exposed to any member of the complex to a varying degree. If the sample is positive using specific criteria set by Coppe Laboratories, confirmation is done by IFA. IFA is performed by incubating patient samples on slides with virus-infected cells and then staining with fluorescein-labeled anti-human antibodies. Positive staining is visualized with a fluorescent microscope.