Test: Powassan Virus EIA/IFA
Indirect Testing: Powassan Serology Test (Serology)
Measurement of patient’s antibody response to the virus
- IgG and IgM antibodies specific for Powassan detected
- Test is useful for determining stage of infection including chronic disease
The EIA measures IgG and IgM antibodies to members of the tick-borne encephalitis complex (TBE-C). Due to the homology between these viruses, antibodies that cross-react with the TBE virus substrate in the EIA kit are found in samples from patients exposed to any member of the complex to a varying degree. If the sample is positive using specific criteria set by Coppe Laboratories, confirmation is done by IFA. IFA is performed by incubating patient samples on slides with virus-infected cells and then staining with fluorescein-labeled anti-human antibodies. Positive staining is visualized with a fluorescent microscope.