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Lyme Tests – Antibodies to Borrelia

Test: Lyme Tests – Antibodies to Borrelia

Overview

  • 3001 – Lyme Borrelia Screening Test (Total Antibody [IgG/IgM] EIA)
  • 3002 – Lyme Confirmatory Tests (IgG and IgM Line Blots)
  • 3003 – Comprehensive Lyme Panel (includes both tests)
Testing for:
Lyme Disease
Tested on:
Plasma, Whole Blood, Serum
Test Codes:
3001, 3002, 3003
  • Uses

    The CDC currently recommends a two-step process when testing for evidence of antibodies against the Lyme disease bacteria, Borrelia. Both steps can be done using the same sample. Coppe Laboratories employs this two-step approach, while using recombinant VlsE and OspC antigens to increase test sensitivity.  The Coppe Lyme panel tests for multiple strains of Borrelia burgdorferi, the European species B. garinii and B. afzelii, and will also detect infections caused by B. miyamotoi.  Testing is recommended 4-6 weeks after initial tick bite.

    Methodology

    Coppe Labs’ screening test uses an enzyme immunoassay (EIA). If screen results are negative, no further testing is recommended. If the screen results are positive or equivocal, confirmatory line blot tests will be performed.

    Lyme Screen – Total Antibody [IgG/IgM] Enzyme Immunoassay (EIA)

    • Includes Borrelia burgdorferi purified OspC antigens
    • Detects infections with multiple strains of Borrelia burgdorferi as well as B. garinii, B. afzelii and B. miyamotoi

    Confirmatory Test – Anti-Borrelia IgG and IgM Line Blots

    • Comprehensive array of diagnostically relevant Borrelia antigens
      • Recombinant VlsE from three genospecies: B. burgdorferi, B. garinii and B. afzelii
      • Immunogenic lipids from B. burgdorferi and B. afzelii upregulated during late stage disease
      • Native OspC from all three genospecies
    • Densitometry software is used for band analysis for objective results

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    Preferred Whole blood to be processed to plasma by Coppe Laboratories
    6 – 10 mL collected in ACD. EDTA also acceptable
    3 mL
    Room temperature less than 72 hours
    Room temperature

    Alternate Plasma: Whole blood collected in ACD or EDTA, processed to plasma
    1 mL
    0.5 mL
    Refrigerator 6 days; frozen 1 year
    If frozen, send on dry ice. If refrigerated, send with ice packs

    Alternate Serum: Whole blood collected in SST and centrifuged. Serum carefully removed
    1 mL
    0.5 mL
    Refrigerator 6 days; frozen 1 year
    If frozen, send on dry ice. If refrigerated, send with ice packs

    Specimen:
    Preferred Whole blood to be processed to plasma by Coppe Laboratories
    Volume / Collection Type:
    6 – 10 mL collected in ACD. EDTA also acceptable
    Minimum:
    3 mL
    Storage:
    Room temperature less than 72 hours
    Shipping:
    Room temperature
    Specimen:
    Alternate Plasma: Whole blood collected in ACD or EDTA, processed to plasma
    Volume / Collection Type:
    1 mL
    Minimum:
    0.5 mL
    Storage:
    Refrigerator 6 days; frozen 1 year
    Shipping:
    If frozen, send on dry ice. If refrigerated, send with ice packs
    Specimen:
    Alternate Serum: Whole blood collected in SST and centrifuged. Serum carefully removed
    Volume / Collection Type:
    1 mL
    Minimum:
    0.5 mL
    Storage:
    Refrigerator 6 days; frozen 1 year
    Shipping:
    If frozen, send on dry ice. If refrigerated, send with ice packs