Diagnostic Test Menu

ciHHV-6 Screening Panel

Test: ciHHV-6 Screening Panel

Overview

  • real-time DNA PCR (qPCR) on Whole Blood
  • reverse transcription PCR for mRNA
  • real-time DNA PCR on hair follicle
Testing for:
CiHHV-6
Tested on:
Whole Blood, Hair Follicle
Test Codes:
1012
  • Methodology

    Real-time DNA PCR

    DNA present in the cellular sample is extracted using a commercial kit. To amplify the small amounts of relevant viral DNA present, viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.

    Reverse Transcription PCR (RT-PCR) for mRNA

    The viral mRNA is extracted from the whole blood sample using a commercial kit. The RT-PCR method is used to detect the expressed viral gene target by reverse transcribing the mRNA of interest into its DNA complement (cDNA). Subsequently, the newly synthesized cDNA is amplified using traditional real-time PCR methods.