Diagnostic Test Menu

ciHHV-6 Screening Panel

Test: ciHHV-6 Screening Panel

Overview

  • real-time DNA PCR (qPCR) on Whole Blood
  • reverse transcription PCR for mRNA
  • real-time DNA PCR on hair follicle
Testing for:
CiHHV-6
Tested on:
Whole Blood, Hair Follicle
Test Codes:
1012
  • Methodology

    Real-time DNA PCR

    DNA present in the cellular sample is extracted using a commercial kit. To amplify the small amounts of relevant viral DNA present, viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.

    Reverse Transcription PCR (RT-PCR) for mRNA

    The viral mRNA is extracted from the whole blood sample using a commercial kit. The RT-PCR method is used to detect the expressed viral gene target by reverse transcribing the mRNA of interest into its DNA complement (cDNA). Subsequently, the newly synthesized cDNA is amplified using traditional real-time PCR methods.

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    Whole blood
    6 – 10 mL collected in ACD tube
    3 mL
    Less than 48 hours from collection to arrival in lab, room temperature
    Room temperature

    Hair follicle
    3-4 hairs with follicle
    At least 1 follicle
    Room temperature
    Room temperature

    Nail clipping
    clippings from ten nails
    3-4 nail clippings
    Room temperature
    Room temperature

    Specimen:
    Whole blood
    Volume / Collection Type:
    6 – 10 mL collected in ACD tube
    Minimum:
    3 mL
    Storage:
    Less than 48 hours from collection to arrival in lab, room temperature
    Shipping:
    Room temperature
    Specimen:
    Hair follicle
    Volume / Collection Type:
    3-4 hairs with follicle
    Minimum:
    At least 1 follicle
    Storage:
    Room temperature
    Shipping:
    Room temperature
    Specimen:
    Nail clipping
    Volume / Collection Type:
    clippings from ten nails
    Minimum:
    3-4 nail clippings
    Storage:
    Room temperature
    Shipping:
    Room temperature
  • Causes for Rejection

    • Specimens submitted in the improper tube or container
    • Specimens not accompanied by a requisition or incomplete requisition
    • Unlabeled specimens
    • Leaking containers
    • Insufficient quantity of specimen
    • Improper storage
    • Failure to receive samples prior to expiration

    Turn Around Time

    Results are available within five business days of receipt of specimen in the lab

    Results

    Qualitative Results

    Report as Detected / Not Detected

    Qualitative Results for RT-PCR for mRNA and DNA PCR on Hair Follicle

    Report as Detected / Not Detected