Test: ciHHV-6 Screening Panel
Real-time DNA PCR
DNA present in the cellular sample is extracted using a commercial kit. To amplify the small amounts of relevant viral DNA present, viral “gene of interest” specific primers and probes, deoxyribonucleotides, and a thermo-stable DNA polymerase are added to the PCR reaction.
Reverse Transcription PCR (RT-PCR) for mRNA
The viral mRNA is extracted from the whole blood sample using a commercial kit. The RT-PCR method is used to detect the expressed viral gene target by reverse transcribing the mRNA of interest into its DNA complement (cDNA). Subsequently, the newly synthesized cDNA is amplified using traditional real-time PCR methods.