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Babesia DNA qPCR on Whole Blood Samples

Test: Babesia DNA qPCR on Whole Blood Samples

Overview

Testing for:
Babesia
Sample Type:
Whole Blood
Test Code/s:
3005
  • Uses

    Real time polymerase chain reaction (PCR) is one of the many variants of PCR used in molecular diagnostics. Real time PCR represents a sensitive and powerful tool for diagnosis and monitoring of acute and chronic infectious agents in patient samples.

    Methodology

    This procedure detects the presence of Babesia 18S rRNA genes in human whole blood. Total nucleic acids are purified from the sample. Conserved sequences of Babesia genes of interest are amplified using genus-specific primers. The presence of amplicon allows a TaqMan probe to bind and be cleaved during replication. As amplicon quantity increases, the fluorescent signal increases. If the sample is positive the fluorescent signal will pass a pre-determined threshold. The cycle at which the fluorescence passes the threshold is the cycle threshold (Ct). The Ct value is compared to a standard curve of synthetic double-stranded DNA (DSD) Ct values to determine a relative quantity of Babesia DNA in the sample. If no amplicon is created, then no probe will be cleaved providing no fluorescent signal. Calibrator, medium positive, low positive and negative control reactions are performed with each test.

  • Specimen

    Volume / Collection Type

    Minimum

    Storage

    Shipping


    Whole blood
    6 – 10 mL collected in ACD tube. EDTA also acceptable
    3 mL
    Room temperature for < 48 hours
    Room temperature

    Specimen:
    Whole blood
    Volume / Collection Type:
    6 – 10 mL collected in ACD tube. EDTA also acceptable
    Minimum:
    3 mL
    Storage:
    Room temperature for < 48 hours
    Shipping:
    Room temperature
    Note: Specimens must be received by lab before expiration of storage times.
  • Turn Around Time

    Results available within four business days of specimen receipt in the lab.

    Results

    Quantitative Results

    Reported as copies/mL and log copies/mL

    Extra Information

    All specimens must be labeled with the patient’s name and collection date. Specimens are accepted Monday through Friday NOON (Central). Call for prior arrangements beyond these accepted times.