Test: Anaplasma IgG / IgM IFA
Human anaplasmosis, formerly known as human granulocytic ehrlichiosis (HGE), is a bacterial disease transmitted to humans by Ixodes scapularis (blacklegged tick or deer tick), the same tick that transmits Lyme disease. Anaplasmosis is a challenging disease for healthcare providers to diagnose based on clinical presentation alone. The symptoms vary from patient to patient and can be difficult to distinguish from other tick-borne diseases, so laboratory testing is critical for a definitive diagnosis.
The gold standard serologic test for diagnosis of anaplasmosis is the indirect immunofluorescence assay (IFA). This assay uses human cells infected with Anaplasma phagocytophilum fixed on 12 well slides and incubated with diluted patient samples. If the sample is positive, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and can be visualized using a fluorescent microscope.